ABSTRACT
Spillage of cyst contents during surgical operation is the major cause of recurrence after hydatid cyst surgery. Instillation of a scolicidal agent into a hepatic hydatid cyst is the most commonly employed measure to prevent this complication. SB202190 is a pyridinyl imidazole derivative and is known to be a specific inhibitor of p38 MAPK. In the present study, the scolicidal effect of SB202190 was investigated. Freshly isolated Echinococcus granulosus protoscolices were subjected to SB202190 treatment (10, 20, 40, and 80 microM), and the effects on parasite viability were monitored by trypan blue staining. Corresponding effects were visualized by scanning and transmission electron microscopy. Dose-dependent protoscolex death within a few days of SB202190 treatment was observed. Although the in vitro scolicidal effect of SB202190 was satisfactory, the in vivo efficacy of this drug and also possible side effects remain to be further investigated.
Subject(s)
Animals , Anthelmintics/pharmacology , Dose-Response Relationship, Drug , Echinococcus granulosus/drug effects , Imidazoles/pharmacology , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Parasitic Sensitivity Tests , Pyridines/pharmacology , Survival AnalysisABSTRACT
Purpose:To study the role of p38MAPK in mediating TNF ? induced apoptosis in rat glioma cells C6.Methods:The proliferation activity of C6 cells after the treatment by TNF ? was observed by MTT assay. The TNF ? induced apoptosis was detected by transmission electron microscopy and flow cytometry. The expression of p38MAPK was detected by SABC method and Westernblot. The effect of SB202190, a specific inhibitor of p38MAPK on TNF ? induced apoptosis was observed by flow cytometry and SABC method. Results:The inhibitory rate of TNF ? (2?10 5 U/L) on C6 cells was 43.75%. In the TNF ? treated group, apoptotic cells were observed by transmission electron microscopy and the apoptotic rate was 37.5% by flow cytometry, p38MAPK positive signals were found by SABC method and Westernblot. In the SB202190 treated group, the apoptotic rate was 7.0% and no p38MAPK signals were found.Conclusions:The apoptosis of C6 cells and expression of p38MAPK could be induced by TNF ?. The activation of p38MAPK promoted the apoptosis of C6 cells. [